RESUMO
INTRODUCTION: We describe the clinical and laboratorial features of oral candidiasis in 66 HIV-positive patients. METHODS: Polymerase chain reaction-based techniques were performed for differentiation of Candida spp. isolated from patients at a public teaching hospital in Midwest Brazil. RESULTS: Oral lesions, mainly pseudomembranous, were significantly related to higher levels of immunosuppression. Of 45 Candida isolates, 66.7% were C. albicans. Most of the isolates were susceptible to the antifungal drugs tested. CONCLUSIONS: Oral lesions were associated with higher immunosuppression levels. Lower susceptibility to antifungals by non-albicans isolates supports the importance of surveillance studies using susceptibility tests to aid in the treatment.
Assuntos
Infecções Oportunistas Relacionadas com a AIDS/diagnóstico , Antifúngicos/farmacologia , Candida/efeitos dos fármacos , Candidíase Bucal/diagnóstico , Infecções Oportunistas Relacionadas com a AIDS/microbiologia , Adulto , Anfotericina B/farmacologia , Brasil , Candida/classificação , Candida/isolamento & purificação , Candidíase Bucal/microbiologia , Resistência Microbiana a Medicamentos , Feminino , Fluconazol/farmacologia , Humanos , Itraconazol/farmacologia , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Técnicas de Tipagem Micológica , Adulto JovemRESUMO
Abstract INTRODUCTION We describe the clinical and laboratorial features of oral candidiasis in 66 HIV-positive patients. METHODS: Polymerase chain reaction-based techniques were performed for differentiation of Candida spp. isolated from patients at a public teaching hospital in Midwest Brazil. RESULTS: Oral lesions, mainly pseudomembranous, were significantly related to higher levels of immunosuppression. Of 45 Candida isolates, 66.7% were C. albicans. Most of the isolates were susceptible to the antifungal drugs tested. CONCLUSIONS: Oral lesions were associated with higher immunosuppression levels. Lower susceptibility to antifungals by non-albicans isolates supports the importance of surveillance studies using susceptibility tests to aid in the treatment.
Assuntos
Humanos , Masculino , Feminino , Adulto , Adulto Jovem , Candida/efeitos dos fármacos , Candidíase Bucal/diagnóstico , Infecções Oportunistas Relacionadas com a AIDS/diagnóstico , Antifúngicos/farmacologia , Brasil , Candida/isolamento & purificação , Candida/classificação , Candidíase Bucal/microbiologia , Resistência Microbiana a Medicamentos , Testes de Sensibilidade Microbiana , Fluconazol/farmacologia , Anfotericina B/farmacologia , Técnicas de Tipagem Micológica , Infecções Oportunistas Relacionadas com a AIDS/microbiologia , Itraconazol/farmacologia , Pessoa de Meia-IdadeRESUMO
Abstract INTRODUCTION Incidence and antifungal susceptibility of Candida spp. from two teaching public hospitals are described. METHODS The minimum inhibitory concentrations of fluconazole, voriconazole, itraconazole, and amphotericin B were determined using Clinical Laboratory Standard Institute broth microdilution and genomic differentiation using PCR. RESULTS Of 221 Candida isolates, 50.2% were obtained from intensive care unit patients; 71.5% were recovered from urine and 9.1% from bloodstream samples. Candida parapsilosis sensu stricto was the most common candidemia agent. CONCLUSIONS We observed variations in Candida species distribution in hospitals in the same geographic region and documented the emergence of non-C. albicans species resistant to azoles.
Assuntos
Humanos , Masculino , Feminino , Recém-Nascido , Lactente , Pré-Escolar , Adolescente , Adulto , Adulto Jovem , Candida/efeitos dos fármacos , Candidíase/microbiologia , Antifúngicos/farmacologia , Brasil , Candida/classificação , Candida/genética , Testes de Sensibilidade Microbiana , Fluconazol/farmacologia , Anfotericina B/farmacologia , Itraconazol/farmacologia , Farmacorresistência Fúngica , Voriconazol/farmacologia , Hospitais Públicos , Pessoa de Meia-IdadeRESUMO
INTRODUCTION: Incidence and antifungal susceptibility of Candida spp. from two teaching public hospitals are described. METHODS: The minimum inhibitory concentrations of fluconazole, voriconazole, itraconazole, and amphotericin B were determined using Clinical Laboratory Standard Institute broth microdilution and genomic differentiation using PCR. RESULTS: Of 221 Candida isolates, 50.2% were obtained from intensive care unit patients; 71.5% were recovered from urine and 9.1% from bloodstream samples. Candida parapsilosis sensu stricto was the most common candidemia agent. CONCLUSIONS: We observed variations in Candida species distribution in hospitals in the same geographic region and documented the emergence of non-C. albicans species resistant to azoles.
